| Autor: |
Meng, Xinhao, Lou, Hongyao, Zhai, Shanshan, Zhang, Runqi, Liu, Guoyu, Xu, Weiya, Yu, Jiazheng, Zhang, Yufeng, Ni, Zhongfu, Sun, Qixin, Xing, Jiewen, Li, Baoyun |
| Předmět: |
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| Zdroj: |
Plant, Cell & Environment; Jun2024, Vol. 47 Issue 6, p2310-2321, 12p |
| Abstrakt: |
Grain protein content (GPC) is a crucial quality trait in bread wheat, which is influenced by the key transcription factor TaNAM. However, the regulatory mechanisms of TaNAM have remained largely elusive. In this study, a new role of TaNAM was unveiled in regulating nitrogen remobilisation which impacts GPC. The TaNAM knockout mutants generated by clustered regularly interspaced short palindromic repeats/Cas9 exhibited significantly delayed senescence and lower GPC, while overexpression of TaNAM‐6A resulted in premature senility and much higher GPC. Further analysis revealed that TaNAM directly activates the genes TaNRT1.1 and TaNPF5.5s, which are involved in nitrogen remobilisation. This activity aids in the transfer of nitrogen from leaves to grains for protein synthesis. In addition, an elite allele of TaNAM‐6A, associated with high GPC, was identified as a candidate gene for breeding high‐quality wheat. Overall, our work not only elucidates the potential mechanism of TaNAM‐6A affecting bread wheat GPC, but also highlights the significance of nitrogen remobilisation from senescent leaves to grains for protein accumulation. Moreover, our research provides a new target and approach for improving the quality traits of wheat, particularly the GPC. Summary statement: Grain protein content (GPC), a trait that affects both food processing quality and nutritional value, is a decisive factor for measuring the commercial value of new wheat varieties. Here, new results were revealed including: (1) We identified new targets of TaNAM‐6A, TaNRT1.1, and TaNPF5.5s, which are indispensable for nitrogen remobilisation from leaves to grains, especially during grain filling; (2) We identified an elite allele in natural populations, TaNAM‐6A‐Hap1, for wheat GPC that could be used in the genetic improvement of wheat quality. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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