| Autor: |
Wang, Yueqiang, Wang, Zhenyu, Liu, Wenhui, Xie, Shuoqi, Ren, Xiaoli, Yan, Lei, Liang, Dong, Gao, Tengyun, Fu, Tong, Zhang, Zhen, Huang, Hetian |
| Předmět: |
|
| Zdroj: |
Genes; Apr2024, Vol. 15 Issue 4, p412, 12p |
| Abstrakt: |
Simple Summary: Ketosis (KET), a metabolic disorder frequently observed in dairy cows, is characterized by increased levels of ketone bodies. The "gold standard" for diagnosing ketosis is the concentration of blood β-hydroxybutyrate (BHB). The increasing number of studies focusing on BHB highlights the increasing significance of metabolic disorders in both the dairy industry and the scientific community. Additionally, the surge in research on the genetic and economic facets of the KET is likely correlated with the recent accessibility of extensive datasets containing blood BHB concentrations as routinely recorded traits in certain production systems. Such data are essential to accurately estimating the genetic parameters associated with these traits. Consequently, the objective of this study was to estimate genetic parameters and identify candidate genes related to blood BHB through genome-wide association analysis to provide research directions for dairy ketosis. Ketosis is a common metabolic disorder in the early lactation of dairy cows. It is typically diagnosed by measuring the concentration of β-hydroxybutyrate (BHB) in the blood. This study aimed to estimate the genetic parameters of blood BHB and conducted a genome-wide association study (GWAS) based on the estimated breeding value. Phenotypic data were collected from December 2019 to August 2023, comprising blood BHB concentrations in 45,617 Holstein cows during the three weeks post-calving across seven dairy farms. Genotypic data were obtained using the Neogen Geneseek Genomic Profiler (GGP) Bovine 100 K SNP Chip and GGP Bovine SNP50 v3 (Illumina Inc., San Diego, CA, USA) for genotyping. The estimated heritability and repeatability values for blood BHB levels were 0.167 and 0.175, respectively. The GWAS result detected a total of ten genome-wide significant associations with blood BHB. Significant SNPs were distributed in Bos taurus autosomes (BTA) 2, 6, 9, 11, 13, and 23, with 48 annotated candidate genes. These potential genes included those associated with insulin regulation, such as INSIG2, and those linked to fatty acid metabolism, such as HADHB, HADHA, and PANK2. Enrichment analysis of the candidate genes for blood BHB revealed the molecular functions and biological processes involved in fatty acid and lipid metabolism in dairy cattle. The identification of novel genomic regions in this study contributes to the characterization of key genes and pathways that elucidate susceptibility to ketosis in dairy cattle. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
