| Autor: |
Curone, Giulio, Filipe, Joel, Inglesi, Alessia, Bronzo, Valerio, Pollera, Claudia, Comazzi, Stefano, Draghi, Susanna, Piccinini, Renata, Ferlazzo, Gianluca, Quattrone, Alda, Vigo, Daniele, Amadori, Massimo, Riva, Federica |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; Apr2024, Vol. 11 Issue 4, p166, 16p |
| Abstrakt: |
Simple Summary: In the dairy industry, bovine mastitis poses significant challenges due to production losses and treatment costs. Understanding innate immunity in bovine milk could be crucial for fighting intra-mammary infections. We conducted cytofluorimetric analyses to assess bacterial killing activity in bovine skim milk against Gram-positive (S. aureus) and Gram-negative (E. coli) pathogens. We found that milk exhibited higher bacterial killing activity against S. aureus compared to E. coli, and those results correlated with N-acetyl-β-D-glucosaminidase (NAGase) levels. Both S. aureus killing and NAGase activity decreased with lactation duration. Milk samples from autochthonous Modenese cows displayed stronger killing activity compared to samples from cosmopolitan breed Holstein Friesian cows. Our findings suggest that skim milk is endued with distinct control mechanisms against different pathogens in the mammary gland, and provide insights into innate immune responses and potential strategies for managing mastitis in the dairy industry, which could lead to improved production efficiency and reduced treatment costs. In the dairy industry, bovine mastitis represents a major concern due to substantial production losses and costs related to therapies and early culling. The mechanisms of susceptibility and effective response to intra-mammary infections are still poorly understood. Therefore, we investigated innate immunity in acellular bovine skim milk through cytofluorimetric analyses of bacterial killing activity against both Gram-positive and Gram-negative pathogens. Freshly cultured E. coli and S. aureus strains were incubated with colostrum and milk samples at different lactation time points from two groups of cows, purportedly representing mastitis-resistant and mastitis-susceptible breeds; bacterial cells were analyzed for vitality by flow cytometry following incorporation of vital dyes. N-acetyl-β-D-glucosaminidase (NAGase) activity was also investigated in milk and colostrum samples. Our findings revealed that colostrum and milk bacterial killing activity was greater against S. aureus compared to E. coli., with this activity correlated with milk NAGase levels. Furthermore, both killing of S. aureus and NAGase activity were negatively correlated to the elapsed time of lactation. Interestingly, samples from the allegedly mastitis-resistant breed displayed higher bacterial killing and NAGase activities. Our study suggests that diverse control mechanisms are exerted against Gram-positive and Gram-negative pathogens in the mammary glands of cows, probably beyond those already described in the literature. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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