| Abstrakt: |
Centaurea perrottetii, which is commonly known as star thistle, belongs to the compositae family and it is used in traditional medicine to treat malaria, microbial infections among others. This study aimed to isolate and characterize compounds from the whole plant of C. perrottetii. The whole plant extract of Centaurea perrottetii was collected, dried, pulverized and extracted with methanol using maceration method. The extract was concentrated in vacuo with the aid of rotary evaporator to afford a greenish crude methanol extract (CME). The extract was successively partitioned into hexane, chloroform, ethylacetate and n-butanol fractions respectively. These fractions were subjected to preliminary phytochemical investigation using standard procedures. A combination of silica gel and sephadex LH-20 column chromatography was employed which led to the isolation of two methoxylated flavones, 8,4'-dihydroxy-6,7,3'-trimethoxyflavone and 3',4'-dihydroxy-6,7,8-trimethoxyflavone, from the ethylacetate soluble fraction of the methanol extract of the whole plant of Centaurea perrottetii. The identity of the compounds was determined via chemical tests, spectroscopic techniques and by comparison with existing data in the literature. To the best of our knowledge, this is the first report of isolation of these compounds from the whole plant of C. perrottetii. [ABSTRACT FROM AUTHOR] |
