| Abstrakt: |
The purpose of this study was to assess the antibacterial activity of an ethanolic extract of Orchis mascula against clinically isolated human pathogenic bacteria, as well as to characterise its structural properties. The ethanolic extract was analysed for quantitative photochemical composition and the chemical constituents were analysed using GC–MS. The functional characterization of ethanolic extract was analysed through FT-IR. Furthermore, of the 10 pathogens tested, the ethanolic extract demonstrated antibacterial activity against 7 clinical bacterial strains. The ethanolic extract had a maximum inhibition zone of 20 mm against Salmonella paratyphi at the highest concentration (100 g/ml). The MIC values for ethanolic extract against bacterial strains such as Salmonella typhi, S. paratyphi, Staphylococcus aureus, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli and Vibrio parahaemolyticus were 80, 40, 100, 100, 100, 100 and 100 μg/ml. The MBC values for ethanolic extract against bacterial strains such as S. typhi, S. paratyphi, S. aureus, V. cholerae, K. oxytoca, E. coli and V. parahaemolyticus were 80, 40, 100, 100, 100, 100 and 100 μg/ml. The effective bacterial inhibition rate of O. mascula suggests that it could be a potential antibacterial agent. [ABSTRACT FROM AUTHOR] |
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