| Autor: |
Li, Yanjiao, Wang, Yunhao, Vera-Rodriguez, Maria, Lindeman, Leif Christopher, Skuggen, Linda Ellevog, Rasmussen, Erik M. K., Jermstad, Ingunn, Khan, Shaista, Fosslie, Madeleine, Skuland, Trine, Indahl, Marie, Khodeer, Sherif, Klemsdal, Eva Kristine, Jin, Kang-Xuan, Dalen, Knut Tomas, Fedorcsak, Peter, Greggains, Gareth D., Lerdrup, Mads, Klungland, Arne, Au, Kin Fai |
| Zdroj: |
Nature Biotechnology; Apr2024, Vol. 42 Issue 4, p591-596, 6p |
| Abstrakt: |
Current N6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos. A picogram-scale m6A mapping method is applied to single mouse oocytes and embryos in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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