Ganoderma spore lipid ameliorates docetaxel, cisplatin, and 5-fluorouracil chemotherapy-induced damage to bone marrow mesenchymal stem cells and hematopoiesis.
| Autor: | Lin, Haohui, Chung, Manhon, Sun, Jingchun, Yang, Yi, Zhang, Li, Pan, Xiaohua, Wei, Minghui, Cai, Sa, Pan, Yu |
|---|---|
| Předmět: |
DOCETAXEL
SQUAMOUS cell carcinoma IN vitro studies FLOW cytometry CISPLATIN BONE marrow DATA analysis RESEARCH funding MESENCHYMAL stem cells HEAD & neck cancer APOPTOSIS CELL proliferation SPORES IN vivo studies REVERSE transcriptase polymerase chain reaction DESCRIPTIVE statistics PLANT extracts CANCER chemotherapy HEMATOPOIESIS MICE REACTIVE oxygen species ANIMAL experimentation WESTERN immunoblotting ANALYSIS of variance STATISTICS FLUOROURACIL CELL survival CYCLOPHOSPHAMIDE PHENOTYPES PHARMACODYNAMICS |
| Zdroj: | BMC Complementary Medicine & Therapies; 4/12/2024, Vol. 24 Issue 1, p1-12, 12p |
| Abstrakt: | Background: A triplet chemotherapy regimen of docetaxel, cisplatin, and 5-fluorouracil (TPF) is used to treat head and neck squamous cell carcinoma; however, it is toxic to bone marrow mesenchymal stem cells (BMSCs). We previously demonstrated that Ganoderma spore lipid (GSL) protect BMSCs against cyclophosphamide toxicity. In this study, we investigated the protective effects of GSL against TPF-induced BMSCs and hematopoietic damage. Methods: BMSCs and C57BL/6 mice were divided into control, TPF, co-treatment (simultaneously treated with GSL and TPF for 2 days), and pre-treatment (treated with GSL for 7 days before 2 days of TPF treatment) groups. In vitro, morphology, phenotype, proliferation, senescence, apoptosis, reactive oxygen species (ROS), and differentiation of BMSCs were evaluated. In vivo, peripheral platelets (PLTs) and white blood cells (WBCs) from mouse venous blood were quantified. Bone marrow cells were isolated for hematopoietic colony-forming examination. Results: In vitro, GSL significantly alleviated TPF-induced damage to BMSCs compared with the TPF group, recovering their morphology, phenotype, proliferation, and differentiation capacity (p < 0.05). Annexin V/PI and senescence-associated β-galactosidase staining showed that GSL inhibited apoptosis and delayed senescence in TPF-treated BMSCs (p < 0.05). GSL downregulated the expression of caspase-3 and reduced ROS formation (p < 0.05). In vivo, GSL restored the number of peripheral PLTs and WBCs and protected the colony-forming capacity of bone marrow cells (p < 0.05). Conclusions: GSL efficiently protected BMSCs from damage caused by TPF and recovered hematopoiesis. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
| Externí odkaz: |
|