| Autor: |
Kratzl, Franziska, Urban, Marlene, Pandhal, Jagroop, Shi, Mengxun, Meng, Chen, Kleigrewe, Karin, Kremling, Andreas, Pflüger-Grau, Katharina |
| Předmět: |
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| Zdroj: |
Communications Biology; 4/12/2024, Vol. 7 Issue 1, p1-14, 14p |
| Abstrakt: |
In their natural habitats, microbes rarely exist in isolation; instead, they thrive in consortia, where various interactions occur. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which supplies sucrose to the heterotrophic P. putida cscRABY, is investigated to identify potential interactions. Initial experiments reveal a remarkable growth-promoting effect of the heterotrophic partner on the cyanobacterium, resulting in an up to 80% increase in the growth rate and enhanced photosynthetic capacity. Vice versa, the presence of the cyanobacterium has a neutral effect on P. putida cscRABY, highlighting the resilience of pseudomonads against stress and their potential as co-culture partners. Next, a suitable reference process reinforcing the growth-promoting effect is established in a parallel photobioreactor system, which sets the basis for the analysis of the co-culture at the transcriptome, proteome, and metabolome levels. In addition to several moderate changes, including alterations in the metabolism and stress response in both microbes, this comprehensive multi-OMICs approach strongly hints towards the exchange of further molecules beyond the unidirectional feeding with sucrose. Taken together, these findings provide valuable insights into the complex dynamics between both co-culture partners, indicating multi-level interactions, which can be employed for further streamlining of the co-cultivation system. This study investigates interspecies interaction in a synthetic co-culture consisting of the sucrose-secreting cyanobacterium S. elongatus cscB and its sucrose-consuming partner P. putida cscRABY by employing a comprehensive multi-OMICs approach. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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