| Abstrakt: |
Two methods for increasing the degree of hydrophilization and shielding of a resin substrate based on a styrene–divinylbenzene copolymer with attached polyethylenimine quaterinized with glycidol are proposed. The first method is the polymerization of glycidol in a functional layer by varying the pH of the reaction medium, and the second one is the modification of the substrate by oxidizing double bonds on its surface to form anchor epoxy groups. It is demonstrated that, in the first case, the optimal approach is to add glycidol twice before and after adding an alkali, because, in this case, the first addition of glycidol is consumed for the quaternization of the polyamine, and the second addition—for the polymerization in ion-exchange centers. The novel method of substrate modification in combination with the developed method for creating hydrophilic layers made it possible to significantly reduce the retention of oxohalides, haloacetic acids, and polarizable anions in the suppressed ion chromatography mode and amino acids in hydrophilic interaction liquid chromatography mode up to a change in the elution order. The obtained stationary phases are suitable for the simultaneous determination of standard inorganic anions, oxohalides, and haloacetic or alkylphosphonic acids in an ion chromatography mode, and also for the separation of amino acids, sugars, and vitamins in a hydrophilic interaction liquid chromatography mode. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink