| Autor: |
Zhang, Huihui, Yao, Yao, Shang, Rujie, Cheng, Yushuai, Li, Guorui, Yu, Hailong, Pang, Jinhui, Li, Lu |
| Zdroj: |
Catalysis Letters; May2024, Vol. 154 Issue 5, p2220-2230, 11p |
| Abstrakt: |
Immobilization is a crucial method for enzyme recovery and utilization. Among all techniques, physical adsorption stands out as a commonly employed immobilization method, driven by the surface properties of the supporter. In this study, the focus is to investigate the effect of the nature of the supporter surface on the performance of the immobilized enzyme. A range of supporters with varying hydrophilic-hydrophobic properties was obtained by treating cover glasses with piranha etching solution and different silane coupling agents. Subsequently, cellulase was immobilized on these modified supporters for cellulose hydrolysis, aiming to investigate the supporter's impact on the activity and stability of immobilized enzymes. Additionally, the supporters were characterized using infrared spectroscopy, contact angle measurements, and Zeta potential analysis. The results indicate that the stronger the supporter's hydrophobicity, the more pronounced the hydrolytic efficiency of immobilized cellulase. This work provides an effective strategy for utilizing supporter surface properties in the context of physical adsorption. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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