| Abstrakt: |
This study aimed to prepare aqueous Dianthus caryophyllus L extract and test its anticancer activity against MCF-7 cells (MCF-7 is a breast cancer cell line) and normal WRL68 cells (WRL68 is normal liver cell line), as well as test its antimicrobial activity on Pseudomonas aeruginosa bacteria isolated from festering human wounds at Salah al-Din General Hospital. 80 swabs were collected from suppurating wounds of people exposed to bruises and accidents in hospital for the period from January to May of 2021. Pseudomonas aeruginosa bacteria were isolated 37 times from patients, which represents 46.25 percent of all bacterial isolates isolated from hospitalized patients. Then, using the well-agar diffusion method, tested the effectiveness of the aqueous extract of Dianthus caryophyllus in inhibiting the growth of these bacteria, five concentrations were tried: 10 mg/ml, 25 mg/ml, 50 mg/ml, and 75 mg/ml. ml, and 100 mg/ml, and the efficacy was gradually increased with increasing concentration, the concentration of 100 mg/ml caused the largest inhibition area for Pseudomonas aeruginosa bacteria on the dish in vitro, and these bacteria were identified by biochemical assays and validated by Vitek-test 2. The anti-cancer efficacy was tested with aqueous Dianthus caryophyllus extract as MCF-7 breast cancer cells had an LD50 of 39.09 percent, while normal WRL68 cells had an LD50 of 4.775 percent, according to the MTT test. The components of the aqueous extract of Dianthus caryophyllus were also determined using GC\MS and FTIR techniques, The results of the GC\MS assays showed that the highest percentage of the supporting substances was for eugenol, which has mainly anti-microbial and anti-carcinogenic activity. [ABSTRACT FROM AUTHOR] |
