| Abstrakt: |
Objective: To investigate the effect of interleukin-12 (IL-12) on liver lesions and oxidative stress pathway in Sjogren's syndrome mice, and to clarify the possible mechanism of liver lesions in Sjogren's syndrome. Methods: Saliva flow rate, liver function, liver pathology and IL-12 level were measured in NOD, IL-12 knockout (IL-12KO) NOD and C57BL/6 (B6) mice. Different concentrations of IL-12 and JAK2, TYK2 inhibitors were applied to mouse hepatoma cells. The oxidative stress index of mouse serum and cell culture supernatants of each group were determined. Results: Compared with the other two groups of mice, the NOD mice had significantly higher GOT and GPT (P<0.05), and decreased serum GSH-PX, SOD and CAT(P<0.05). CAT, GSH, GSH-PX and SOD were decreased, while MDA was increased in mice treated with different concentrations of IL-12. JAK2/TYK2 inhibitors reversed regulatory effects of IL-12 on SOD, GSH and MDA in hepatoma cells (P<0.05). Conclusion: IL-12 may aggravated liver damage of Sjögren's syndrome through promoting oxidative stress of hepatocytes. [ABSTRACT FROM AUTHOR] |
