| Autor: |
Salinas, Julio, Zerial, Marino, Filipski, Jan, Bernardi, Giorgio |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 11/3/86, Vol. 160 Issue 3, p469-478, 10p |
| Abstrakt: |
Mouse DNA was fractionated by preparative centrifugation in density gradients of CS2SO4 containing 3,6-bis(acetatomercurimethyl)dioxane (BAMD). The effects of temperature, BAMD/nucleotide molar ratio and solvent on the fractionation were explored. The fractions so obtained were investigated by analytical centrifugation in CsCl density gradient and by hybridization will a number of gene probes. These approaches led (a) to the definition of satisfactory conditions for the rapid fractionation of mouse DNA; (b) to the localization of a number of genes in mouse DNA fractions; and (c) to a better understanding of the mosaic organization of the mouse genome and, more specifically, to a better estimate of both the intermolecular and intramolecular compositional heterogeneity of mouse DNA in the (75-150) × 10-base size range. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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