| Abstrakt: |
Introduction: Obesity leads to the development of cardiovascular disease (CVD) which is the main cause of death in postmenopausal women. Dietary interventions such as calorie restriction (CR) is the most efficient to reduce fat mass in obese individuals and improve plenty of the metabolic complications associated with obesity, including CVD. Therefore, the effects of calorie restriction and 17-beta estradiol on cardiac hypertrophy in ovariectomized obese rats was studied. Methods and Materials: Adult female Wistar rats were categorized into two different groups, Sham and OVX (ovariectomized) groups, and received a standard diet (SD), high-fat diet (60%HFD), or underwent 30% CR for a period of 16 weeks. Then, OVX animals were administered 17-β estradiol (E2) at a dosage of 1 mg/kg intraperitoneally (ip) every four days for 4 weeks. SIRT1 protein expression was determined by the ELISA method. Hematoxylin and eosin staining was conducted to observe the pathological changes of cardiomyocytes diameter. Results: HFD consumption resulted in a reduction in SIRT1 protein expression (P<0.001), an increment in heart weight/body weight ratio (P<0.01), and cardiomyocyte diameter (P<0.05) in OVX rats. In contrast, E2 and CR increased SIRT1 protein expressions (P<0.01 and P<0.05; respectively), reduced heart weight/body weight ratios (P<0.05 and P<0.01; respectively) and cardiomyocytes diameter (P<0.01 and P<0.05; respectively) in these animals. Conclusion: The current study demonstrated that CR and E2 are able to reduce obesity-induced cardiac hypertrophy in postmenopausal conditions by acting through the mediation of SIRT1. [ABSTRACT FROM AUTHOR] |
