| Abstrakt: |
Introduction: Medicinal plants possess numerous compounds that can be used for biosynthesis of gold nanoparticles (AuNPs). It has been reported that Camellia sinensis, Salvia officinalis, Rosmarinus officinalis, and Matricaria chamomilla possess various biological properties, including antioxidant and anti-cancer activities. The present study was conducted to assess the antioxidant activities and anticancer effects of biosynthesized AuNPs applying aqueous extracts of CS, SO, RO, and MC against two human breast cancer cell lines. Methods and Materials: The formation of Phyto-NPs was characterized by TEM and UV-vis spectroscopy. The antiproliferative activities and cytotoxic effects of the aqueous extracts and the aqueous extracts containing AuNPs on the MCF-7, MCF-10, and MDA-MB-231 cell lines were examined using MTT assay. The antioxidant potential of all four aqueous leaf/flower extracts and Phyto-AuNPs was analyzed by FRAP and DPPH assays. Results: UV–vis spectrum of SO-AuNPs, RO-AuNPs, CS-AuNPs, and MC- AuNPs displayed absorption peaks at wavelengths of 520±6 nm, 540±8 nm, 520±7 nm, and 530±9 nm, respectively. TEM image showed an almost spherical shape of Phyto-AuNPs with particle sizes of 60-80 nm. Phyto-AuNPs have demonstrated enhanced antioxidant activities in comparison with the plant extracts. Furthermore, Phyto-AuNPs also showed higher cytotoxic activities against MCF-7 and MDA-MB-231 cell lines than the plant extracts. Conclusion: AuNPs synthesized using CS, RO, SO, and MC aqueous leaf/flower extracts displayed significant cytotoxic eff ects on MCF-7 and MDA-MB-231 cell lines, suggesting that they could be a potential antitumor agent in the treatment of metastatic breast cancer. However, further studies are required to explain the molecular mechanisms involved and to test them in experimental breast cancer models in animals. [ABSTRACT FROM AUTHOR] |
