| Autor: |
میترا مهربانی, سعیده جعفری نژاد, محبوبه رئیس زاده, مژده اسماعیلی طر, مژگان شیخ الاسلا, محمدهادی نعمت ال, وجبهه خوش فکر, کبری بهرام پور جو, مهرناز مهربانی |
| Předmět: |
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| Zdroj: |
Koomesh: Journal of Semnan University of Medical Sciences; Sep/Oct2023, Vol. 25 Issue 5, p608-608, 2p |
| Abstrakt: |
Introduction: Over recent years, much attention has been devoted to the area of screening natural products and/or their novel structures owing to reversing cancer progression. The present research work was planned to investigate the cytotoxic activity of ethanol and ethyl acetate extracts of dried fruit of Terminalia chebula Retz. (T. chebula) in MCF-7 cell line. Methods and Materials: To determine the total phenolic contents and to identify the main compounds in both extracts, Folin-Ciocalteu colorimetric and high-performance thin-layer chromatographic (HPTLC) techniques were used, respectively. Anti-proliferative properties of T. chebula fruit extracts on the MCF-7 cell line were assessed using MTT assay. Effects of both extracts on the migration of MCF-7 cells and the size of MCF-7-derived spheroids were also evaluated. Moreover, the antioxidant activities of both extracts were assessed by DPPH and FRAP methods. Western blotting was used to evaluate the HIF-1α and CXCR-4 protein levels. Results: Chebulagic acid, gallic acid, chebulinic acid, and ellagic acid were found as the main compounds in both extracts. The total phenolic contents based on gallic acid equivalent (GAE) in the ethanol and ethyl acetate extracts of T. chebula were found to be 453.68±0.31 and 495.12±0.43 mg GAE/g dry weight of the extract, respectively. Both extracts exhibited significant dose- and time-dependent cytotoxic effects on MCF-7 cells. They also had a marked negative effect on the average size of MCF-7-derived spheroids and their migration rate. None of the extracts exhibited stronger antioxidant activities than vitamin C. Furthermore, both extracts at a concentration of 125 µg/ml could significantly reduce the HIF-1α and CXCR-4 protein levels in MCF-7 cells. Conclusion: These data suggest that T. chebula may be a valuable medicinal resource in the management of breast cancer proliferation, growth, and metastasis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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