| Autor: |
غزل دهقان دهکردی, حسن صدرائی, افسانه یگدانه, مائده قاسمی |
| Předmět: |
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| Zdroj: |
Koomesh: Journal of Semnan University of Medical Sciences; Sep/Oct2023, Vol. 25 Issue 5, p443-443, 2p |
| Abstrakt: |
Introduction: Thymus daenensis Celak (T.daenensis) is one of the endogenous Thymus species in Iran. The plant is traditionally used as carminative, digestive, antispasmodic and expectorant in Iran. It has been claimed to have antispasmodic activities, but only limited studies have been done to support this. Therefore, the objective of this research was to investigate anti-spasmodic effect of T.daenensis extracts and essential oil on uterine contractions. Methods and Materials: Hydroalcoholic extract was prepared using the maceration technique. In addition, hexane fraction was prepared using a solvent in solvent fractionation technique. Essential oils were collected using the hydrodistillation technique. Rat isolated uterus was suspended in an organ bath and contracted with acetylcholine (ACh) and KCl. The relaxant effects of the extract, its fractions, essential oil and nifedipine were examined on uterine contractions induced by the above-mentioned stimuli. Uterine contractions were recorded on Harvard Universal Oscillographs. Effect of the extract or the essential oil were determined by adding directly into the organ bath. The control tissues were treated with equivolume of the extract vehicle. Nifedipine was used as standard drug. Uterine contractions were measured as amplitude of the contraction and expressed as percentage of recorded contractions prior to addition of drug. Results: Nifedipine in a concentration-dependent manner inhibited uterine contraction induced by KCl and ACh. T.daenensis extracts also exhibited an inhibitory effect on the isolated rat uterus. Comparison of the hydroalcoholic extract with its hexane fractions showed that the hexane fraction was more potent than the hydroalcoholic extract. Moreover, the relaxant effect of essential oil was much more potent than hydroalcoholic and hexane fractions. Conclusion: According to the results, the lipophilic compounds of the hexane partition seem to be the main cause of the antispasmodic effects of the T.daenensis extracts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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