| Abstrakt: |
Introduction: Kaempferol is a flavonoid with pharmacological effects, including antinociceptive and antiinflammatory properties. GABAB receptors are as inflammatory mediators in the possible antinociception. This study aimed to investigate the involvement of GABAB receptors in the possible antinociceptive activity of Kaempferol in rats. Methods and Materials: In this experimental study 11 groups of adults male Wistar rats (200-250 g), (n=5) were used. The groups were: DMSO group, saline group, naloxan group, morphine(10 μg/rat) group, kaempferol at dosages of 0.5, 1, and 1.5 mg/rat groups, baclofen group, CGP35348 group, baclofen plus kaempferol (1 mg/rat) group and CGP 35348 plus kaempferol (1 mg/rat) group. Rats have been surgically treated and the cannula was implanted into the right brain ventricle and after a recovery week the desired compounds (DMSO, saline, naloxone, morphine, Kaempferol at dosages of 0.5, 1, and 1.5 mg/rat, baclofen and CGP35348) were injected via the cannula into the ventricle and the rats were subjected to nociception test, Includes formalin test (Index: pain score). Baclofen was used as the GABAB receptor agonist, Kaempferol as antinociceptive and CGP35348 as an antagonist of the GABAB receptor. A rotarod test was also carried out to assess motor function. Results: Kaempferol reduced the pain score index in the late phase of the formalin test. Adminisration of both Kaempferol (1mg/rat) plus baclofen had significant analgesic effects in comparison to the Kaempferol group (1 mg/rat) at the formalin test. Injection of both Kaempferol (1 mg/rat) plus CGP35348 decreased antinociceptive effects of Kaempferol group (1 mg/rat) alone at the pain assessment tests. Conclusion: These findings propose Kaempferol antinociceptive effects through GABAB receptors and inflammatory mechanisms in male rats. [ABSTRACT FROM AUTHOR] |
