| Abstrakt: |
Introduction: Fluoxetine is a selective serotonin reuptake inhibitor (SSRI) used in the treatment of neurological disorders such as depression, bulimia nervosa, and obsessive-compulsive disorder. Among all SSRIs, fluoxetine has a high clinical use due to its safety and effectiveness. However, based on past studies, fluoxetine leads to a decrease in viability in various cells such as ovarian epithelial, hippocampal cells, and liver cells through increasing oxidative stress. Since many depressed men are at reproductive age and need long-term use of fluoxetine to treat their depression, it is necessary to investigate its effects on sperm cell quality. The aim of this study is to evaluate, the effect of fluoxetine on the motility, viability, morphology, and total antioxidant capacity (TAC) of human sperm in vitro. Methods and Materials: Semen samples from 30 normal fertile men were collected and divided into three groups: fresh, control (without treatment and 1-hour incubation), and fluoxetine (treated with 5 µM fluoxetine and 1-hour incubation). The motility (total, progressive, non-progressive), viability, morphology, TAC level, and ROS level in the sperm were evaluated. Data were analyzed using repeated measures analysis and considered significantly different at p<0.05. Results: The mean percentage of total motility, progressive motility, viability, normal morphology, and sperm TAC level showed a significant decrease in the fluoxetine group compared to the fresh and control groups (p˂0.001). A significant increase in the mean percentage of non-progressive mobility and ROS level was observed in the fluoxetine group compared to the fresh and control groups (p˂0.001). Conclusion: Fluoxetine led to a decrease in TAC level and an increase in ROS level in sperm which disturbs the sperm quality and the male reproductive health [ABSTRACT FROM AUTHOR] |
