| Autor: |
Maiuri, Luigi, Ciacci, Carolina, Vacca, Loredana, Ricciardelli, Ida, Auhcchio, Salvatore, Quaratino, Sonia, Londei, Marco |
| Předmět: |
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| Zdroj: |
American Journal of Gastroenterology (Springer Nature); Jan2001, Vol. 96 Issue 1, p150-156, 7p, 2 Color Photographs, 1 Graph |
| Abstrakt: |
OBJECTIVES: Celiac disease (CD) is an under-diagnosed but extremely frequent disease, triggered by the ingestion of gliadin. The pathogenic mechanisms of CD are still poorly understood, but intraepithelial lymphocytes are considered to have a key role. We intended to define the subsets of T lymphocytes migrating upon gliadin challenge in organ cultures of treated celiac patients and establish the type of factor(s) driving such an infiltration. METHODS: Duodenum biopsies from 10 treated celiacs and 7 controls were cultured in vitro with/without gliadin digest (1 mg/ml) or interleukin (IL)-15 (10 ng/ml). In 7 treated celiacs IL-7, lL-4, and lL-2 were similarly tested, Intraepithelial CD3, CDS, TCR-γδ, and CD94 were detected by immunohistochemistry and numbered per mm epithelium. RESULTS: TL-15 but not IL-7, IL-4, or IL-2 induced intra-epithelial increase of CD3+ and CD8+ cells in celiac and control intestine (p < 0.001 vs cultures with medium). IL-15 induced increases in the number of intraepithelial TCR-γδ+ and CD94+ cells only in celiacs (p < 0.001). IL-7 was also effective in increasing intraepithelial TCR-γδ+ (but not CD94+) cells in celiac biopsies (p < 0.001), Gliadin induced intraepithelial migration of CD3 + , CD8+ (p < 0.001), and CD94+ (p < 0.05) cells in celiacs, but not in controls. CONCLUSIONS: The results we describe in this report indicate that IL-15 might have a key role in modulating and driving intraepithelial infiltration and ultimately in the pathogenesis of CD. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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