Beta-Sitosterol Alters Collagen Distribution in Prostate Fibroblasts.
| Autor: | D'Arcy, Quentin, Sarna-McCarthy, Marissa, Bowen, Delaney, Soto, Fidias O., Zarringhalam, Kourosh, Macoska, Jill A. |
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| Předmět: |
RNA analysis
PROSTATE physiology MINERAL analysis IN vitro studies CANCER ENZYME-linked immunosorbent assay PROSTATE tumors REVERSE transcriptase polymerase chain reaction FLUORESCENT antibody technique IN vivo studies DESCRIPTIVE statistics PHYTOSTEROLS FIBROBLASTS PROSTATE FIBROSIS EPITHELIUM VITAMINS WESTERN immunoblotting COLLAGEN CELL survival CANCER patient psychology COMPARATIVE studies DATA analysis software DIETARY supplements IMMUNOBLOTTING |
| Zdroj: | Journal of Dietary Supplements; 2024, Vol. 21 Issue 3, p313-326, 14p |
| Abstrakt: | Herbal supplements containing several types of plant sterols, vitamins, and minerals, are marketed for prostate health. In the majority of these supplements, the most abundant plant sterol is saw palmetto extract or its' principal component, beta-sitosterol. In terms of prostate health, previous work almost exclusively focused on the effects of beta-sitosterol on prostatic epithelium, with little attention paid to the effects on prostatic stroma. This omission is a concern, as the abnormal accumulation of collagen, or fibrosis, of the prostatic stroma has been identified as a factor contributing to lower urinary tract symptoms and dysfunction in aging men. To address whether beta-sitosterol may be promoting prostatic fibrosis, immortalized and primary prostate stromal fibroblasts were subjected to immunoblotting, immunofluorescence, qRT-PCR, ELISA, and image quantitation and analysis techniques to elucidate the effects of beta-sitosterol on cell viability and collagen expression and cellular localization. The results of these studies show that beta-sitosterol is nontoxic to prostatic fibroblasts and does not stimulate collagen production by these cells. However, beta-sitosterol alters collagen distribution and sequesters collagen within prostatic fibroblasts, likely in an age-dependent manner. This is a significant finding as prostate health supplements are used predominantly by middle aged and older men who may, then, be affected disproportionately by these effects. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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