| Autor: |
Castro, Alexander D., Franco Rodríguez, Jorge E., Adarme López, María C. |
| Předmět: |
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| Zdroj: |
Revista Colombiana de Ciencias Pecuarias; 2024 Supplement, Vol. 37, p157-157, 1/2p |
| Abstrakt: |
Introduction: salmonellosis is a global zoonotic disease with widespread distribution. Antibiotic resistance poses a significant threat to global health and food safety. Inappropriate antibiotic usage in human and veterinary medicine‚ as well as in animal production‚ has led to the emergence of bacteria resistant to multiple antibiotics. This has limited treatment options‚ increasing the morbidity and mortality associated with bacterial infections‚ and becoming a major public health concern. Objective: this study aimed to assess the stability of plasmid-encoded resistance genes without selective pressure and determine the clonal relationships among isolates carrying PMQR genes and their minimum inhibitory concentration (MIC). Materials and methods: fifteen isolates of Salmonella spp. from chicken and pork‚ known to harbor PMQR genes‚ underwent MIC determination using the BD Phoenix™ kit. Specific primers (Eric1‚ Eric2‚ Rep1‚ Rep2) were employed to amplify DNA fragments for clonality analysis‚ visualized on 2 % agarose gels. The similarity was calculated using different metrics‚ and a dendrogram was constructed using the unweighted pair group method with arithmetic mean analysis (UPGMA). Additionally‚ gene tracking was performed by PCR every five generations to verify gene presence or absence. Each isolate underwent 50 replications. Results: the study revealed that isolates carrying PMQR genes were multidrug-resistant (MDR) and exhibited resistance to multiple antibiotics (MRA). Plasmid qnr genes showed high stability until replication 15‚ with only nine isolates amplifying until replication 50. Bacterial typing using ERIC-PCR and REP-PCR techniques demonstrated acceptable discriminatory power‚ although low reproducibility and challenges in analyzing banding patterns due to low intensity were observed. Conclusions: this study highlights the importance of continuous surveillance of antibiotic resistance in foodborne bacteria and emphasizes the need to implement prevention and control strategies to mitigate the risk of bacterial transmission to humans. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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