| Autor: |
Tomris, Ilhan, van der Woude, Roosmarijn, de Paiva Froes Rocha, Rebeca, Torrents de la Peña, Alba, Ward, Andrew B., de Vries, Robert P. |
| Zdroj: |
Protein Science: A Publication of the Protein Society; Apr2024, Vol. 33 Issue 4, p1-13, 13p |
| Abstrakt: |
Enveloped viruses carry one or multiple proteins with receptor‐binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor‐binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N‐ and C‐terminal, for influenza A virus hemagglutinins and SARS‐CoV‐2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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