| Autor: |
Heckmann, Mara, Stadlbauer, Verena, Drotarova, Ivana, Gramatte, Theresa, Feichtinger, Michaela, Arnaut, Verena, Atzmüller, Stefanie, Schwarzinger, Bettina, Röhrl, Clemens, Blank-Landeshammer, Bernhard, Weghuber, Julian |
| Předmět: |
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| Zdroj: |
Antioxidants; Mar2024, Vol. 13 Issue 3, p297, 15p |
| Abstrakt: |
Numerous underexplored plant species are believed to possess considerable potential in combating oxidative stress and its associated health impacts, emphasizing the need for a comprehensive methodological screening approach to assess their antioxidant capacity. This study investigated 375 plant extracts, utilizing both cell-free and cellular methods to evaluate their antioxidant properties. Target-based antioxidant capacity was evaluated by the total phenolic content (TPC) and ferric reducing antioxidant power (FRAP) assays. Cell-based assays employed the H2DCF-DA probe to measure reactive oxygen species (ROS) levels and the Griess assay to quantify nitric oxide (NO) levels in stressed Caco-2 and RAW264.7 cells, respectively. The highest TPC and FRAP values were found in extracts of Origanum vulgare and Fragaria × ananassa leaves. Several plant extracts significantly reduced stress-induced ROS or NO levels by at least 30%. Distinctive selectivity was noted in certain extracts, favoring the significant reduction of NO (e.g., Helianthus tuberosus extract), of ROS (e.g., Prunus domestica subsp. Syriaca extract), or of both (e.g., Fragaria × ananassa leaf extract). A strong correlation between TPC and FRAP values and moderate correlations between the results of the cell-free and cell-based assays were evident. These findings highlight the great antioxidant potential of underexplored plant extracts and the diversity of the underlying mechanisms, emphasizing the importance of a multifaceted approach for a comprehensive assessment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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