| Abstrakt: |
Objective: To explore the effects of N6-methyladenine (m6A) modification-related genes, methyltransferase 14 (METTL14), and YTH domain family protein 1 (YTHDF1), on the proliferation, migration and apoptosis capabilities of cervical cancer cells and investigate their correlation with programmed cell death-ligand 1 (PD-L1) expression. Methods: The expression levels of METTL14, YTHDF1 and PD-L1 in cervical cancer tissues and normal cervical tissues were analyzed using immunohistochemistry. Small interfering RNA (siRNA) was used to knock down the expression of METTL14 and YTHDF1 genes in cervical cancer cells, and the knockdown efficiency was validated by real-time fluorescent quantitative PCR (qPCR). After knockdown of METTL14 and YTHDF1, cell proliferation was assessed by CCK-8 assay, cell migration was examined by Transwell assay, cell apoptosis was detected by flow cytometry, and PD-L1 mRNA and protein expression were evaluated using qPCR and Western blotting, respectively. Results: Immunohistochemistry results demonstrated high expression of METTL14, YTHDF1, and PD-L1 in cervical cancer tissues. Knockdown of METTL14 and YTHDF1 significantly inhibited the proliferation and migration capabilities of cervical cancer cells, increased apoptosis, and downregulated PD-L1 mRNA and protein expression levels. Conclusion: m6A methylation modification can affect the proliferation, migration and apoptosis of cervical cancer cells by regulating the expression of PD-L1 in cervical cancer cells. [ABSTRACT FROM AUTHOR] |
