| Autor: |
ANANYA, NAGESHA, N. |
| Předmět: |
|
| Zdroj: |
Mysore Journal of Agricultural Sciences; Jan-Mar2024, Vol. 58 Issue 1, p30-39, 10p |
| Abstrakt: |
Ridge gourd is cultivated in tropical, subtropical and few varieties like Pusa Nasdar and Satputia temperate regions across the world. It is a rich source of essential vitamins and minerals around the world. However, the production of ridge gourd has been severely affected by diseases among which viral diseases have major impact on its production. The viral disease caused by tomato leaf curl New Delhi virus (ToLCNDV), a member of the genus Begomovirus, family Geminiviridae-an economically important virus affecting the ridge gourd yield. It is transmitted by the whiteflies [Bemisia tabaci (Gennadius)] and causes ridge gourd yellow mosaic disease (RgYMD) in ridge gourds. The present study mainly aimed at the process of development of Agrobacterium coat protein gene construct and in silico analysis of CP. The total DNA from infected leaves of ridge gourd with ToLCNDV was amplified using CP specific primers with amplicon size of 771bp. The purified CP. gene was successfully cloned to the TA cloning vector. The CP gene was further moved into plant expression binary vector pBI121. The gene construct of the plant expression vector was transformed into Agrobacterium tumefaciens. In-silico analysis of the coat protein revealed that the protein contains nuclear export factors BR1 family. BLAST results analysis of coat protein with respect to the other coat proteins of ToLCNDV depicted 80-90 per cent resemblance. Phylogenetic tree revealed the common origin of this protein. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
