Multimode chromatography‐based techniques for high purity isolation of extracellular vesicles from human blood plasma.

Autor: Zimmerman, Alan J., de Oliveira, Getulio Pereira, Su, Xianyi, Wood, Jacqueline, Fu, Zhengxin, Pinckney, Brandy, Tigges, John, Ghiran, Ionita, Ivanov, Alexander R.
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Zdroj: Journal of Extracellular Biology; Mar2024, Vol. 3 Issue 3, p1-15, 15p
Abstrakt: Extracellular vesicles (EVs) play a pivotal role in various biological pathways, such as immune responses and the progression of diseases, including cancer. However, it is challenging to isolate EVs at high purity from blood plasma and other biofluids due to their low abundance compared to more predominant biomolecular species such as lipoprotein particles and free protein complexes. Ultracentrifugation‐based EV isolation, the current gold standard technique, cannot overcome this challenge due to the similar biophysical characteristics of such species. We developed several novel approaches to enrich EVs from plasma while depleting contaminating molecular species using multimode chromatography‐based strategies. On average, we identified 716 ± 68 and 1054 ± 35 protein groups in EV isolates from 100 µL of plasma using multimode chromatography‐ and ultracentrifugation‐based techniques, respectively. The developed methods resulted in similar EV isolates purity, providing significant advantages in simplicity, throughput, scalability, and applicability for various downstream analytical and potential clinical applications. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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