| Autor: |
Wallart, Lisa, Ben Mlouka, Mohamed Amine, Saffiedine, Brahim, Coquet, Laurent, Le, Hung, Hardouin, Julie, Jouenne, Thierry, Phan, Gilles, Kiefer-Meyer, Marie-Christine, Girard, Eric, Broutin, Isabelle, Cosette, Pascal |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 2024, p1-16, 16p |
| Abstrakt: |
Previously, we pointed out in P. aeruginosa PAO1 biofilm cells the accumulation of a hypothetical protein named PA3731 and showed that the deletion of the corresponding gene impacted its biofilm formation capacity. PA3731 belongs to a cluster of 4 genes (pa3732 to pa3729) that we named bac for "Biofilm Associated Cluster." The present study focuses on the PA14_16140 protein, i.e., the PA3732 (BacA) homolog in the PA14 strain. The role of BacA in rhamnolipid secretion, biofilm formation and virulence, was confirmed by phenotypic experiments with a bacA mutant. Additional investigations allow to advance that the bac system involves in fact 6 genes organized in operon, i.e., bacA to bacF. At a molecular level, quantitative proteomic studies revealed an accumulation of the BAC cognate partners by the bacA sessile mutant, suggesting a negative control of BacA toward the bac operon. Finally, a first crystallographic structure of BacA was obtained revealing a structure homologous to chaperones or/and regulatory proteins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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