| Autor: |
Pramasari, Dwi Ajias, Oktaviani, Maulida, Fahrezi, M. Zuvan Maulana, Thontowi, Ahmad, Kanti, Atit, Hermiati, Euis |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2024, Vol. 2973 Issue 1, p1-9, 9p |
| Abstrakt: |
Xylitol is a common sugar replacement used throughout food and pharmaceutical applications as well as a component in a wide range of chemical products. Xylitol could be produced from lignocellulosic biomass through acid or enzymatic hydrolysis, continued by fermentation of the xylose-rich hydrolysate using yeasts. To obtain better results during the fermentation of acid hydrolysate, the yeast should have a good tolerance to acidic environments. For improving the tolerance of Meyerozyma caribbica Y67 to low pH conditions, we suggested a short-term adaptation technique in this work to enhance xylitol production on sugarcane trash hemicellulosic hydrolysate (STHH). This study aims to evaluate the effectiveness of short-term adaptation techniques and various pH in simple shake flasks for Meyerozyma caribbica Y67 yeast for obtaining the higher xylitol. The hydrolysate was prepared by dilute acid hydrolysis using 1.8% (v/v) maleic acid assisted by a microwave digester (1:10 solid/liquid ratio, 180 °C, 5 min, 195 rpm). Yeast was cultivated in YPX broth (pH 5.0) for 24 h to obtain inoculum cells and consecutively transferred to the subsequent YPX broth with variations in pH adjustment (4; 3.5; 3; 2.5) and each was adapted for 72 h. Adapted cells were used as inoculum in fermentations using STHH for 72-h at the same pH as that they were adapted to. Results of the study show that the adapted yeast at pH 2.5 produced the highest xylitol concentration, (0.48 g/L) with a yield (Yp/s) of 0.05 g/g xylose in 72 h compare to other adaptation conditions. Therefore, the short-term adaptation of yeast cells in STHH was demonstrated to be an effective method for enhancing yeast tolerance to the low pH of the acid hydrolysate as well as its fermentative performance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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