| Autor: |
Baulcombe, D., Flavell, R. B., Boulton, R. E., Jellis, G. J. |
| Předmět: |
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| Zdroj: |
Plant Pathology; Sep1984, Vol. 33 Issue 3, p361-370, 10p |
| Abstrakt: |
A DNA copy (cDNA) of the common European strain (strain-group 3) potato virus X RNA (PVX³-RNA) was prepared by reverse transcription of PVX-RNA and amplified by cloning into E. coli plasmid pBR322. This DNA hybridizes with PVX-RNA and not at all to RNA of potato virus Y. A low amount of cross-hybridization was observed with strain-group 2 of PVX (PVX²). However, by hybridization of PVX²-RNA to restriction endonuclease fragments of PVX³-cDNA, it was demonstrated that cross-homology was not present in all regions of the PVX genome. The cloned PVX³-cDNA was hybridized to viral RNA in evade sap samples which had been spotted on to nitrocellulose membrane. The signal, which was assayed by autoradiography, allowed the detection of 50 pg of viral RNA. This value was not adversely affected by encapsidation of the RNA, nor by components of crude sap. The sap spot hybridization was as effective as enzyme-linked immunosorbent assay both in detection of small quantities of purified PVX³, and in screening of plants from a breeding programme for immunity to PVX³ infection. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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