Condensed DNA Structure in Bacteria Subjected to Various Types of Stress.

Autor: Krupyanskii, Y. F., Loiko, N.G., Kovalenko, V. V., Generalova, A. A., Tereshkin, E. V., Moiseenko, A. V., Tereshkina, K. B., Popov, A. N., Sokolova, O. S.
Zdroj: Moscow University Biological Sciences Bulletin; 2023 Suppl 1, Vol. 78, pS45-S49, 5p
Abstrakt: The architecture of DNA in the dormant cells was studied by synchrotron radiation diffraction and transmission electron microscopy. Diffraction experiments indicate the appearance of a periodic ordered organization of DNA. Transmission electron microscopy made it possible to visualize information about the type of DNA condensation. Intracellular nanocrystalline, liquid-crystalline and folded nucleosome-like structures of DNA have been found. Next, we studied changes in DNA architecture under stress of exposure to a chemical analogue of the anabiosis autoinducer (4-hexylresorcinol). Studies of the DNA architecture in the anabiotic and mummified states show the identity of the DNA structure in the anabiotic state and in the dormant state under starvation stress. The architecture of DNA in a mummified state is very different from that of DNA in an anabiotic state. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index