Autor: |
ALI-AROUS, Samir, MEZIANE, Malika, BENTURQUIA, Abdellah Nabil, DJELOUAH, Khaled |
Předmět: |
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Zdroj: |
AGROFOR International Journal; 2024, Vol. 9 Issue 1, p101-107, 7p |
Abstrakt: |
Citrus crop in Chlef valley showed many cases of decline and other typical symptoms of tristeza disease in old and young trees grafted on various rootstock and seedlings acquired from multiple origins in the valley. In order to decipher the reasons of the this citrus quick decline syndrome, a large scale survey of citrus tristeza virus (CTV) and its aphid vectors was carried out from the spring of 2016 to the autumn of 2019 in order to evaluate its current situation in term of distribution and strains. Samples collected from 93 orchards located in 21 localities were tested by DTBIA/DAS-ELISA. The analyses have confirmed the presence of CTV in 54 samples through the study area. Some of the CTV sources were chosen for further molecular genotype characterization associated with the CTV isolates now spreading in the Chlef area. Characterization with multiple molecular markers M.M.M.s and CP gene sequencing showed the presence of the T30 and VT genotypes. This result allowed confirmation of the presence of a virulent strain belonging to the VT genotype. The other CTV isolates were close to those recorded in Mitidja region, with nucleotide identity of 98,6 to 99,1% with the worldwide T30 mild CTV isolates. This early finding of a strain belonging to the severe VT genotype beside to efficient aphid vectors is an issue for Algerian citrus producers and needs rapid actions to be taken by the National phytosanitary services, extending the surveillance to other citrus production regions and uprooting the infected trees. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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