Effect of Deuterium Oxide on Gene Expression of recA and colD Induced by UV Radiation in Esherichia coli Cells.

Autor: Abilev, S. K., Smirnova, S. V., Shapiro, T. N.
Předmět:
Zdroj: Biology Bulletin; Dec2023, Vol. 50 Issue 11, p2997-3002, 6p
Abstrakt: It was shown for the first time that preliminary deuteration of Esherichia coli bacteria with deuterium oxide (D2O) at concentrations from 5 to 10% in a medium leads to an enhancement of the SOS response in the biosensors E. coli MG1655 (pColD::lux) and E. coli MG1655 (pRecA::lux) induced by UV at a dose of 12 J/m2. The biosensors used in this work contain hybrid plasmids carrying the luxCDABE operon of the Photorhabdus luminescens photobacterium, placed under the control of the cda (colD) and recA gene promoters. The induction of genotoxic factors in biosensors indicates the activation of the corresponding E. coli SOS system of gene expression. A simultaneous study of the intensity of luminescence and the cell viability of the E. coli MG1655 biosensor (pCol-lux) bacteria showed that UV irradiation at a dose of 12 J/m2 increases the intensity of luminescence from 2111.1 in a medium without D2O to 5030.3 RLU for 107 viable cells in a medium with D2O. The intensity of luminescence of the biosensor was 2.5 times higher in the medium with deuterium than without it. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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