| Autor: |
Makled, Amal Fathalla, Ghareib, Mohamed Medhat, Ali, Sahar, Sharweed, Mostafa Mahmoud, Sleem, Asmaa shaban |
| Předmět: |
|
| Zdroj: |
Microbes & Infectious Diseases; Feb2024, Vol. 5 Issue 1, p190-200, 11p |
| Abstrakt: |
Background: Staphylococcus epidermidis (S. epidermidis) has emerged as a major etiological factor in implant-related infections, primarily due to its biofilm forming ability. These biofilms enhances bacterial resistance to antimicrobial agents. Aim: This study aimed to detect and characterize drug-resistant S. epidermidis strains in clinical samples obtained from Menoufia University Hospitals, with evaluating their biofilm-forming ability and assessing the antimicrobial activity of chlorhexidine and povidone iodine. Methodology: Drug-resistant S. epidermidis strains were identified using the Vitek2 system and their biofilm producing ability was determined. The presence of biofilmrelated genes (icaA and icaD) was confirmed through polymerase chain reaction (PCR). Multi-drug resistant S. epidermidis isolates capable of biofilm formation were exposed to varying concentrations of chlorhexidine (0.025%, 0.035%, 0.05%, 0.12%) and povidone iodine (1.5%, 3.5%, 7.5%, 10%) for different exposure times. Results: Of 354 clinical isolates, 75 (21.2%) were identified as drug-resistant S. epidermidis using Vitek2 system. Biofilm production was observed and confirmed by the presence of icaA / icaD genes among 61-isolates. Chlorhexidine demonstrated significant effectiveness in vitro at concentration of 0.05% with a short exposure time of 1 minute. In contrast, povidone iodine required higher concentrations and prolonged exposure times to exhibit antibacterial activity. Conclusion: Chlorhexidine was an effective antimicrobial agent against S. epidermidis, particularly when used at clinically available concentrations (0.05%) with short exposure time, whereas povidone-iodine required higher concentrations with prolonged exposure times. Further investigations are warranted to optimize the use of these antiseptics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
