| Autor: |
Dewi, Rizky Aulia Prasasti, Widyasti, Erma, Waltam, Deden Rosid, Wahjono, Edi, Dianursanti, Hasanah, Nuur Faridatun, Setyahadi, Siswa, Aji, Nurul Apsari, Nandyawati, Dewi, Santoso, Eko, Achnafani, Dini, Mufti, Ayi, Pradiv, Molina Indah, Abidin, Kharis Yohan |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2024, Vol. 3080 Issue 1, p1-7, 7p |
| Abstrakt: |
Penicillin G Acylase (PGA) is the responsible enzyme for hydrolysis of β-lactams antibiotics. It became important since the majority process to produce these antibiotics turn into enzymatic. One which is amoxicillin. In this study, a wild type of Bacillus thuringiensis BGSC BD1 among three other isolates from Bioindustrial Lab, LAPTIAB, BRIN Culture Collection are selected as the best producer of the enzyme. The PGA production in LB media for five trials resulting the stable PGA activity of about 3 U/ml with harvesting time at 20 hours of fermentation. The characterization of the enzyme showed optimum activity in a pH range of 7 to 8 with maximum temperature up to 60 °C. The SDS-PAGE result showed two bands with molecular weights around 26 and41 kDa that represent the α and β units of PGA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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