The Cellular Response to Exposure to Ionizing Radiation and Light in the Presence of a Photosensitizer.

Autor: Akulinichev, S. V., Glukhov, S. I., Efremenko, A. V., Kokontsev, D. A., Kuznetsova, E. A., Martynova, V. V., Feofanov, A. V., Yakovlev, I. A.
Zdroj: Biophysics; Oct2023, Vol. 68 Issue 5, p783-791, 9p
Abstrakt: The combined use of X-ray irradiation and photodynamic therapy on a model of human lung adenocarcinoma A549 cells has been investigated. It was found that synchronous exposure to two types of radiation contributed to a stronger decrease in the survival rate of tumor cells than their sequential use or each type of radiation separately. The levels of RNA and expression of a number of genes involved in cell cycle control (p21 and DINOL), apoptosis (BAX and PUMA) and DNA repair (PARP1, OGG1, Rad51, and Lig4) were determined for various combinations of these two types of irradiations of cells. The highest level of expression under all treatments was observed for the long noncoding RNA of DINOL gene. The detected increase in the expression of the BAX and PUMA genes indicated the development of apoptosis. The results we obtained confirmed the synergism of radiation and photodynamic therapy upon their simultaneous use. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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