Cloning and Sequencing of Hepatitis B Virus Pre-S and S Gene Regions.

Autor: Chirara, M. M., Chetsanga, C. J.
Předmět:
Zdroj: Scandinavian Journal of Immunology; Dec92 Supplement 11, Vol. 36, p63-66, 4p
Abstrakt: The polymerase chain reaction (PCR) was used to amplify the pre-S1, pre-S2 and S gene regions of hepatitis B virus (HBV). Sera from three different patients were used as the source of HBV DNA. The resulting 1.2-kb amplification product was cloned into the plasmid pIB130. Restriction enzyme analysis revealed that there are two BamHI sites located about 300 bp apart within the S gene. DNA sequencing revealed a greatest homology to the HBVadw subtype. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index