| Autor: |
Zi-Hong Huang, Chun-Hua Lai, Bang-Yong Wang, Bin-Sheng Luo, Kennelly, Edward James, Qin-Gang Tan |
| Předmět: |
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| Zdroj: |
Traditional Medicine Research; May2024, Vol. 9 Issue 5, p1-10, 10p |
| Abstrakt: |
Background: Trachelospermi Caulis et Folium, the stems and leaves of Trachelospermumjasminoides (Lindl.) Lem, has been used as a remedy for rheumatism, gonarthritis, backacheand pharyngitis, according to the Chinese Pharmacopoeia. However, the correspondingchemical substances of this ethnic medicinal plant with anti-inflammatory activitykept unclear. This study aimed to explore the active substances attribute to its anti-rheumaticactivity of this medical plant. Methods: Ultra-high performance liquid chromatographycombined with quadrupole time-of-flight mass spectrometry was used to identifythechemical components in the extract of T. jasminoides. Molecular docking was appliedtoanalyze the binding affinity of potential active lignans to inducible nitric oxide synthaseenzyme. The anti-inflammatory effects of the potential active fraction were verifiedbycell experiments in vitro. Results: Forty-one compounds, mainly are lignans and nine of whichwere reported for the first time from the genus of Trachelospermum, were characterizedusingultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometrymethod. Molecular docking results showed these identified lignans had excellent interactions with the inducible nitric oxide synthase enzyme, and the lignans-enrichedfraction could significantly inhibit the release of nitric oxide and the expression of induciblenitric oxide synthase protein in LPS-induced RAW264.7 cells. Conclusion: Lignans arethemain active substances that responsible for the anti-inflammatory activity of the ethnicherbal medicine T. jasminoides. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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