Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase.

Autor: Fuji, Saashia, Yamauchi, Shota, Sugiyama, Naoyuki, Kohchi, Takayuki, Nishihama, Ryuichi, Shimazaki, Ken-ichiro, Takemiya, Atsushi
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Zdroj: Nature Communications; 2/20/2024, Vol. 15 Issue 1, p1-13, 13p
Abstrakt: Plasma membrane H+-ATPase provides the driving force for light-induced stomatal opening. However, the mechanisms underlying the regulation of its activity remain unclear. Here, we show that the phosphorylation of two Thr residues in the C-terminal autoinhibitory domain is crucial for H+-ATPase activation and stomatal opening in Arabidopsis thaliana. Using phosphoproteome analysis, we show that blue light induces the phosphorylation of Thr-881 within the C-terminal region I, in addition to penultimate Thr-948 in AUTOINHIBITED H+-ATPASE 1 (AHA1). Based on site-directed mutagenesis experiments, phosphorylation of both Thr residues is essential for H+ pumping and stomatal opening in response to blue light. Thr-948 phosphorylation is a prerequisite for Thr-881 phosphorylation by blue light. Additionally, red light-driven guard cell photosynthesis induces Thr-881 phosphorylation, possibly contributing to red light-dependent stomatal opening. Our findings provide mechanistic insights into H+-ATPase activation that exploits the ion transport across the plasma membrane and light signalling network in guard cells. Light-induced stomatal opening is crucial for photosynthesis. Here the authors show that blue light triggers phosphorylation of two Thr residues in the C-terminal autoinhibitory domain of plasma membrane H+-ATPase, thereby promoting stomatal opening. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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