| Autor: |
KEPES, ZITA, HEGEDUS, EVA, SASS, TAMAS, CSIKOS, CSABA, SZABO, JUDIT P., SZUGYICZKI, VIKTORIA, HAJDU, ISTVÁN, KERTESZ, ISTVAN, OPPOSITS, GABOR, IMREK, JOZSEF, BALKAY, LASZLO, KALMAN, FERENC KRISZTIÁN, TRENCSENYI, GYORGY |
| Předmět: |
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| Zdroj: |
In Vivo; Mar/Apr2024, Vol. 38 Issue 2, p574-586, 13p |
| Abstrakt: |
Background/Aim: Herein we assessed the feasibility of imaging protocols using both hypoxia-specific [18F]F-FAZA and [18F]F-FDG in bypassing the limitations derived from the non-specific findings of [18F]F-FDG PET imaging of tumor-related hypoxia. Materials and Methods: CoCl2-generated hypoxia was induced in multidrug resistant (Pgp+) or sensitive (Pgp-) human ovarian (Pgp- A2780, Pgp+ A2780AD), and cervix carcinoma (Pgp- KB-3-1, Pgp+ KB-V-1) cell lines to establish corresponding tumor-bearing mouse models. Prior to [18F]F-FDG/[18F]F-FAZA-based MiniPET imaging, in vitro [18F]F-FDG uptake measurements and western blotting were used to verify the presence of hypoxia. Results: Elevated GLUT-1, and hexokinase enzyme- II expression driven by CoCl2-induced activation of hypoxiainducible factor-1a explains enhanced cellular [18F]F-FDG accumulation. No difference was observed in the [18F]FFAZA accretion of Pgp+ and Pgp- tumors. Tumor-to-muscle ratios for [18F]F-FAZA measured at 110-120 min postinjection (6.2±0.1) provided the best contrasted images for the delineation of PET-oxic and PET-hypoxic intratumor regions. Although all tumors exhibited heterogenous uptake of both radiopharmaceuticals, greater differences for [18F]FFAZA between the tracer avid and non-accumulating regions indicate its superiority over [18F]F-FDG. Spatial correlation between [18F]F-FGD and [18F]F-FAZA scans confirms that hypoxia mostly occurs in regions with highly active glucose metabolism. Conclusion: The addition of [18F]F-FAZA PET to [18F]F-FGD imaging may add clinical value in determining hypoxic sub-regions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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