| Abstrakt: |
A recent report from the University of Airlangga in Surabaya, Indonesia, discusses the optimization of proteinase K incubation protocols for DNA extraction from oral squamous cell carcinoma (OSCC) samples. The researchers aimed to determine the optimal duration for proteinase K incubation to achieve the highest DNA yields. They divided OSCC samples into three groups and subjected them to different proteinase K incubation protocols. The results showed that the most optimized protocol was 48 hours at room temperature with an additional four hours at 56°C, which resulted in the highest DNA yields. This study highlights the importance of optimizing DNA extraction protocols from formalin-fixed paraffin-embedded (FFPE) samples for molecular biological analysis. [Extracted from the article] |
