| Autor: |
Marques Borges, Gabriel Silva, Santos, Thais Tunes, Pinto, Cristiane Monteiro, Frézard, Frédéric, Blanco, Verônica Freitas, Ondei, Roberta, Rumbelow, Stephen, Miranda Ferreira, Lucas Antônio, Gontijo de Aguiar, Marta Marques, Castro Goulart, Gisele Assis |
| Zdroj: |
Nanomedicine; Feb2024, Vol. 19 Issue 5, p383-396, 14p |
| Abstrakt: |
Aim: To develop nanoemulsions (NEs) loading amphotericin B (AmB) and to evaluate the influence of different excipients on the stability and the supramolecular organization, retention and toxicity of AmB. Materials & methods: The NEs were developed from different oils, surfactants, external media and anionic lipids (disteaoryl phosphatidylglycerol [DSPG] and dioleoyl phosphatidylglycerol [DOPG]). Their impact on the size, pH, zeta potential, AmB encapsulation efficiency, AmB retention and hemolytic potential of the NEs was evaluated. Results & conclusion: The use of soybean oil (lipid matrix), Span 80 (surfactant), phosphate buffer (external phase) and DSPG or DOPG (hydrophobic ion pair) provided better NE stability, higher AmB retention within the NEs and a safer formulation profile in hemolysis tests. Disteaoryl phosphatidylglycerol and dioleoyl phosphatidylglycerol form a hydrophobic ion pair with amphotericin B in nanoemulsions, enhancing drug retention after dispersion and decreasing its toxicity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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