| Abstrakt: |
Objective: To investigate the effect of astragaloside IV on cerebral tissue injury caused by ischemia/reperfusion (I/R) and pyroptosis mediated by Caspase-1 signaling pathway. Methods: SD rats were randomly divided into 4 groups of sham operation (Sham), model (MCAO/R), solvent control (DMSO) and astragaloside IV (AS IV). Cerebral I/R injury model was established by occlusion of the middle cerebral artery for 2 hours and reperfusion for 24 hours. Zea Longa score was used to observe the neurological deficit of rats, the volume of cerebral infarction in rats was evaluate by TTC staining, pathological changes of brain tissue was observed by HE staining, pyroptosis of brain was observed by Caspase-1 and TUNEL immunofluorescence double staining, and protein expressions of NLRP3, Cleaved Caspase-1, IL-18 and IL-1β in brain tissue were detected by Western blot. Results: By constructing the MCAO/R model, our findings suggest that astragaloside IV can reduce the neurological deficit caused by I/R in rats, reduce the infarct volume ofbrain, relieve the necrosis and loss of nerve cells on the infarct side, and inhibit pyroptosis by regulating the classical pyroptosis pathway mediated by Caspase-1. Conclusion: Astragaloside IV may alleviate cerebral I/R injury by inhibiting pyroptosis via regulating the classical pyroptosis pathway mediated by Caspase-1. [ABSTRACT FROM AUTHOR] |
