Immobilization of microbial cells in a sol-gel matrix using a precursor with two non-hydrolyzed bonds.

Autor: Lantsova, Elizaveta A., Kamanina, Olga A., Rybochkin, Pavel V., Arlyapov, Vyacheslav A., Saverina, Evgenia A., Vereshchagin, Anatoly N.
Zdroj: Journal of Sol-Gel Science & Technology; Feb2024, Vol. 109 Issue 2, p310-317, 8p
Abstrakt: In this work, the yeast cells of Ogataea polymorpha BKM Y-2559 were immobilized in a organosilicon sol-gel membrane. It was shown that yeasts immobilized in a matrix containing 85 vol.% diethoxydimethylsilane (DEDMS) and 15 vol.% tetraethoxysilane (TEOS) have the highest activity. Long-term storage of the resulting biohybrid material at a temperature of –18 °С demonstrated that the low temperature does not lead to a drop in the catalytic activity of the immobilized yeast. Maintaining the activity of the yeast encapsulated in the sol-gel matrix is important in the production and storage of commercial biocomposite samples. The study of the structure of the obtained material showed that the yeast cells of Ogataea polymorpha are located in the sol-gel matrix and are not able to be washed out, while the substrates and metabolites can diffuse to the cells without restrictions through the pores in the material. Highlights: Number of matrices with different ratios of diethoxydimethylsilane and tetraethoxysilane were investigated for effective yeast immobilization and further use as a bioreceptor element of a biosensor. Organosilicate matrix is able to protect the resulting biohybrid material at a temperature of –18 °С and preserve the catalytic activity of immobilized yeast Ogataea polymorpha BKM Y-2559. Using the SEM, EDX and BET methods, the structure of the obtained porous material was studied and it was determined that the yeast cells are located in the sol-gel matrix and are not able to be washed out, while the substrates and metabolites can diffuse to the cells without restrictions through the pores in the material. The results of the study confirm the possibility of preserving the activity of yeast encapsulated in a sol-gel matrix, which is important in the production and storage of commercial samples of biocomposites. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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