Transcriptomics Provide Insights into the Photoperiodic Regulation of Reproductive Diapause in the Green Lacewing, Chrysoperla nipponensis (Okamoto) (Neuroptera: Chrysopidae).

Autor: Liu, Shaoye, Gao, Yuqing, Shi, Rangjun, Huang, Haiyi, Xu, Yongyu, Chen, Zhenzhen
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Zdroj: Insects (2075-4450); Feb2024, Vol. 15 Issue 2, p136, 15p
Abstrakt: Simple Summary: Chrysoperla nipponensis (Okamoto) is an important predatory insect, primarily preying on various agricultural pests such as aphids. The green lacewing exhibits facultative diapause, which is primarily influenced by the photoperiod. Although previous research has shown the physiological phenotypic responses of diapause-sensitive and pre-diapause periods to photoperiods in C. nipponensis, the corresponding regulatory mechanisms underlying its diapause remain somewhat unclear. In this study, transcriptome sequencing was performed to explore the molecular basis for diapause, and dsRNA-mediated knockdown was used to elucidate the role of juvenile hormone acid methyltransferase 1 (Jhamt1) in regulating diapause in C. nipponensis. Our findings not only provide insights into the transcriptional regulation processes of genes within diapause-sensitive and pre-diapause periods but also unveil the key genes responsible for regulating diapause in C. nipponensis during the pre-diapause phase. Chrysoperla nipponensis (Okamoto) displays typical adult reproductive diapause under short photoperiods; however, our understanding of the molecular mechanism underlying photoperiod-sensitive reproduction remains limited. In this study, we performed transcriptome profiling of four treatments (the diapause-sensitive stage and pre-diapause phase under long and short photoperiods) of C. nipponensis using RNA sequencing (RNA-seq). A total of 71,654 unigenes were obtained from the samples. Enrichment analysis showed that fatty acid metabolism-related pathways were altered under a short photoperiod. Moreover, β-oxidation-related gene expression was active during the diapause-sensitive period under a short photoperiod. The knockdown of juvenile hormone acid methyltransferase 1 (Jhamt1) prolonged the pre-oviposition period but did not affect the reproductive ability of female individuals in C. nipponensis. These findings provided us with a more comprehensive understanding of the molecular mechanisms of photoperiod-sensitive diapause and show that groundwork is crucial for bolstering the long-term storage and biocontrol potential of C. nipponensis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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