| Abstrakt: |
Simple Summary: Our research focuses on how parasitic worms, specifically Heligmosomoides polygyrus, can change the body's immune response. These parasites can lower the body's defense mechanisms, but exactly how they do this is not fully understood. We believe that a type of immune cell, called macrophages, might be involved. Changes in these cells could potentially help tumors grow. In our study, we looked at how substances produced by H. polygyrus affect these immune cells and, in turn, how they influence the growth of breast cancer cells in the lab. We discovered that these substances from H. polygyrus increase the activity of certain genes in the immune cells. These genes are responsible for both promoting and reducing inflammation. Additionally, these substances change the surface features of the immune cells. Our findings show that these changes in immune cells caused by H. polygyrus could lead to increased growth of breast cancer cells in a laboratory setting. This research helps us understand more about how changes in the immune system can affect tumor growth, which is important for developing new cancer treatments. In our study, we explored how parasitic nematodes, specifically Heligmosomoides polygyrus, influence the immune response, focusing on their potential role in tumor growth. The study aimed to understand the mechanisms by which these parasites modify immune cell activation, particularly in macrophages, and how this might create an environment conducive to tumor growth. Our methods involved analyzing the effects of H. polygyrus excretory-secretory antigens on macrophage activation and their subsequent impact on breast cancer cell lines EMT6 and 4T1. We observed that these antigens significantly increased the expression of genes associated with both pro-inflammatory and anti-inflammatory molecules, such as inducible nitric oxide synthase, TNF-α, (Tumor Necrosis Factor) Il-6 (Interleukin), and arginase. Additionally, we observed changes in the expression of macrophage surface receptors like CD11b, F4/80, and TLR4 (Toll-like receptor 4). Our findings indicate that the antigens from H. polygyrus markedly alter macrophage behavior and increase the proliferation of breast cancer cells in a laboratory setting. This study contributes to a deeper understanding of the complex interactions between parasitic infections and cancer development, highlighting the need for further research in this area to develop potential new strategies for cancer treatment. [ABSTRACT FROM AUTHOR] |
