| Autor: |
Keckstein, Simon, Tilgener, Constantin, Jeschke, Udo, Hofmann, Simone, Vilsmaier, Theresa, Keilmann, Lucia, Heidegger, Helene, Kaltofen, Till, Batz, Falk, Mahner, Sven, Schröder, Lennard |
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| Zdroj: |
Archives of Gynecology & Obstetrics; Apr2024, Vol. 309 Issue 4, p1509-1514, 6p |
| Abstrakt: |
Purpose: In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods: MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results: The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion: MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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