Chromosomal analysis of Vanda celebica, V. dearei, and their hybrids orchid.

Autor: Hartati, S., Sukaya, Samanhudi, Agatha, B.
Předmět:
Zdroj: AIP Conference Proceedings; 2024, Vol. 3001 Issue 1, p1-8, 8p
Abstrakt: The Vanda Orchid is a genus of orchids belongs to the Vandaceous group. The diversity of flower colors and various variations of the Vanda Orchid causes Vanda to be in great demand by fans to be used as ornamental plants and cut flowers. One benefit of using orchid plants to create novel hybrids is that they are versatile. The purpose of this investigation was: (1) to ascertain genetic information in the form of the number, size, and shape of chromosomes in V. celebica, V. dearei, and the results of hybrid V. celebica x V. dearie. (2) to determine the karyotype pattern of V. celebica, V. dearei, and the results of the cross of V. celebica x V dearei. The research was carried out at the Genetics Laboratory and the Cytology Laboratory of the Biological Research Center (LIPI) Bogor. Orchid plant meristematic root tips were employed as the raw material using the squash method for chromosome observation. Chromosomal analysis was performed using the asymmetry index of chromosomes A1 (chromosomal shape) and A2 (chromosomal deviation) in one karyotype. The findings revealed the chromosomal count in total for V. celebica 2n = 38, V. dearei 2n = 40, and the combination of V. celebica x V. dearei 2n = 38. The average length of the chromosomes of V. celebica was 1.09 ± 0.35 µm, V. dearei 1.64 ± 0.33 µm, and V. celebica x V. dearei 1.84 ± 0.36µm. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index