Protective Effects of Danmu Extract Syrup on Acute Lung Injury Induced by Lipopolysaccharide in Mice through Endothelial Barrier Repair.

Autor:	Xu, Han, Xu, Si-cong, Li, Li-yan, Wu, Yu-huang, Tan, Yin-feng, Chen, Long, Liu, Pei, Liang, Chang-fu, He, Xiao-ning, Li, Yong-hui
Předmět:	LIPOPOLYSACCHARIDES LUNG injuries PROTEINS CYTOKINES INTERLEUKINS HERBAL medicine ENDOTHELIUM BRONCHOALVEOLAR lavage ANIMAL experimentation INFLAMMATION WESTERN immunoblotting CELLULAR signal transduction TUMOR necrosis factors ENZYME-linked immunosorbent assay PHOSPHOPROTEINS EPITHELIAL cells MEMBRANE proteins VASCULAR endothelial growth factors OXIDOREDUCTASES ACUTE diseases CHINESE medicine MICE THERAPEUTICS
Zdroj:	Chinese Journal of Integrative Medicine; Mar2024, Vol. 30 Issue 3, p243-250, 8p
Abstrakt:	Objective: To investigate the effects of Danmu Extract Syrup (DMS) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and explore the mechanism. Methods: Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table (n=12), including control (normal saline), LPS (5 mg/kg), LPS+DMS 2.5 mL/kg, LPS+DMS 5 mL/kg, LPS+DMS 10 mL/kg, and LPS+Dexamethasone (DXM, 5 mg/kg) groups. After pretreatment with DMS and DXM, the ALI mice model was induced by LPS, and the bronchoalveolar lavage fluid (BALF) were collected to determine protein concentration, cell counts and inflammatory cytokines. The lung tissues of mice were stained with hematoxylin-eosin, and the wet/dry weight ratio (W/D) of lung tissue was calculated. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1 β in BALF of mice were detected by enzyme linked immunosorbent assay. The expression levels of Claudin-5, vascular endothelial (VE)-cadherin, vascular endothelial growth factor (VEGF), phospho-protein kinase B (p-Akt) and Akt were detected by Western blot analysis. Results: DMS pre-treatment significantly ameliorated lung histopathological changes. Compared with the LPS group, the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment (P<0.05 or P<0.01). The number of cells in BALF and myeloperoxidase (MPO) activity decreased significantly after DMS pretreatment (P<0.05 or P<0.01). DMS pre-treatment decreased the levels of TNF-α, IL-6 and IL-1 β (P<0.01). Meanwhile, DMS activated the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) pathway and reversed the expressions of Claudin-5, VE-cadherin and VEGF (P<0.01). Conclusions: DMS attenuated LPS-induced ALI in mice through repairing endothelial barrier. It might be a potential therapeutic drug for LPS-induced lung injury. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink