Inflammatory and Immunomodulatory Effects of Tripterygium wilfordii Multiglycoside in Mouse Models of Psoriasis Keratinocytes.

Autor:	Zhang, Shuo, Li, Hong-jin, Yang, Chun-mei, Liu, Liu, Sun, Xiao-ying, Wang, Jiao, Chen, Si-ting, Lu, Yi, Hu, Man-qi, Yan, Ge, Zhou, Ya-qiong, Miao, Xiao, Li, Xin, Li, Bin
Předmět:	INFLAMMATION prevention PSORIASIS BIOLOGICAL models QUINOLINE CYTOKINES IN vitro studies FLOW cytometry REVERSE transcriptase polymerase chain reaction INTERLEUKINS MEDICINAL plants IN vivo studies ANTI-inflammatory agents ANIMAL experimentation SKIN inflammation IMMUNE system GLYCOSIDES LYMPH nodes SEVERITY of illness index NEUTROPHILS GENE expression RESEARCH funding HISTOLOGICAL techniques CELL proliferation MESSENGER RNA TUMOR necrosis factors PLANT extracts STATISTICAL sampling T cells CHEMOKINES KERATINOCYTES MICE PHARMACODYNAMICS EVALUATION
Zdroj:	Chinese Journal of Integrative Medicine; Mar2024, Vol. 30 Issue 3, p222-229, 8p
Abstrakt:	Objective: To determine the role of Tripterygium wilfordii multiglycoside (TGW) in the treatment of psoriatic dermatitis from a cellular immunological perspective. Methods: Mouse models of psoriatic dermatitis were established by imiquimod (IMQ). Twelve male BALB/c mice were assigned to IMQ or IMQ+TGW groups according to a random number table. Histopathological changes in vivo were assessed by hematoxylin and eosin staining. Ratios of immune cells and cytokines in mice, as well as PAM212 cell proliferation in vitro were assessed by flow cytometry. Pro-inflammatory cytokine expression was determined using reverse transcription quantitative polymerase chain reaction. Results: TGW significantly ameliorated the severity of IMQ-induced psoriasis-like mouse skin lesions and restrained the activation of CD45+ cells, neutrophils and T lymphocytes (all P<0.01). Moreover, TGW significantly attenuated keratinocytes (KCs) proliferation and downregulated the mRNA levels of inflammatory cytokines including interleukin (IL)-17A, IL-23, tumor necrosis factor α, and chemokine (C-X-C motif) ligand 1 (P<0.01 or P<0.05). Furthermore, it reduced the number of γ δ T17 cells in skin lesion of mice and draining lymph nodes (P<0.01). Conclusions: TGW improved psoriasis-like inflammation by inhibiting KCs proliferation, as well as the associated immune cells and cytokine expression. It inhibited IL-17 secretion from γ δ T cells, which improved the immune-inflammatory microenvironment of psoriasis. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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